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New Generation Reproductive Technologies (stem Cell-nanotechnology)
I. Advances in Embryonic and Placental Development
1.Abstract:
Early embryonic development needs detailed investigations if we have to reduce embryonic mortality. Embryonic mortality details have been reviewed (wani, 2005). The reproductive abnormalities have been discussed and a sequential photographic presentation of embryonic mortality have recently been published (Khatoon et al 2007). The critical period when maximum embryonic losses go unnoticed is the period before attachment. Losses before actual union of foeto-maternal tissues can be missed and calculations of embryonic mortality estimates remain unaccounted for. Some recent studies of (Wani 2006, Khatoon et al 2006) have paved a new innovative model for investigation on foeto-maternal development and its attachment. This paper describes the detailed embryonic development, blastocyst elongation, formation of cotyledons within elongated blastocyst, their ramifications and networking of vasculature and formation of foetal placental unit. Conversely, a detailed photographic evidence of uterine receptivity, formation of a multicoated immunological barrier- the beginning of foeto- maternal- barrier mechanism along with caruncle formation, uterine milk formation and finally union of cotyledous with caruncles has been photographically documented.
2.Introduction:
Second and 3rd generation animal biotechnologies as reviewed (Wani, 2005) are multiple ovulation, embryo-transfer, preservation, cloning, micromanipulation splitting of embryos and nuclear transfer. The 4th Generation reproductive technologies have advance beyond our imagination and now we do have transgenic goats giving milk rich in insulin. (Wani 2007). The stem cell technology, embryonic cell culture, pronuclear micro-injection along with sperm-mediated, transgenesis have been in use and are precisely reviewed and discussed (Wani 2005, 2007).
The application of this advancement for diagnostic, therapeutic and economic uses especially its transfer to human medicine or for field application in animal industry needs further research and evaluation.
The another fact which can be of importance is the number of cells per embryos. During first day these may be two cells but after 8 days the cell number increases to 120 or more. It takes 32 hours for the oocyst after fertilization to reach the two cell embryo. Another 13 hours are needed to develop it into a four cell embryo. In another 15 hours 8 cell stages is reached. It is from here onwards that it takes 30 hours for a embryo to be converted into a Morula. This has been one of the most critical periods of embryonic development in ovine. A genematic diagram of various stages of follical maturation of oocytes development and further development has been reported (Wani 2005a) figure from web site is also show that the protein content after fertilization of 16 day old embryo increased by 7500 times. Morphologically the embryo size varies from spherical ovid to elongate after day 13th past coitus. New protein synthesis per unit of embryo is maximum during 8-13th day old embryos. Similarly phosphorylation per unit of embryo is also ten times more in 8 day old embryos than the later stages. Glucose and lactose production increased up to day 16th and the maximum metabolic weight is up to 14th day past coitus. For increased productive synthesis, aminoacid requirements are made, through production of allanic and glutonic acids by the embryos. The matobolic products get rid of amines. The signal transduction from maternal to foetal controls have been discussed (Moris et al 2001).
3. Dominant follicle dynamics
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